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Endometrial stem cell differentiation into smooth muscle cell: a novel approach for bladder tissue engineering in women
Author(s) -
ShoaeHassani Alireza,
Sharif Shiva,
Seifalian Alexander M.,
MortazaviTabatabaei Seyed Abdolreza,
Rezaie Sassan,
Verdi Javad
Publication year - 2013
Publication title -
bju international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.773
H-Index - 148
eISSN - 1464-410X
pISSN - 1464-4096
DOI - 10.1111/bju.12195
Subject(s) - hepatocyte growth factor , vascular endothelial growth factor , stem cell , tissue engineering , biology , microbiology and biotechnology , biomedical engineering , medicine , cancer research , vegf receptors , receptor , biochemistry
Objective To investigate manufacturing smooth muscle cells ( SMCs ) for regenerative bladder reconstruction from differentiation of endometrial stem cells ( EnSCs ), as the recent discovery of EnSCs from the lining of women's uteri, opens up the possibility of using these cells for tissue engineering applications, such as building up natural tissue to repair prolapsed pelvic floors as well as building urinary bladder wall.Materials and Methods Human EnSCs that were positive for cluster of differentiation 146 ( CD 146), CD 105 and CD 90 were isolated and cultured in D ulbecco's modified E agle/ F 12 medium supplemented with myogenic growth factors. The myogenic factors included: transforming growth factor β, platelet‐derived growth factor, hepatocyte growth factor and vascular endothelial growth factor. Differentiated SMCs on bioabsorbable polyethylene‐glycol and collagen hydrogels were checked for SMC markers by real‐time reverse‐transcriptase polymerase chain reaction ( RT‐PCR ), western blot ( WB ) and immunocytochemistry ( ICC ) analyses.Results Histology confirmed the growth of SMCs in the hydrogel matrices. The myogenic growth factors decreased the proliferation rate of EnSCs , but they differentiated the human EnSCs into SMCs more efficiently on hydrogel matrices and expressed specific SMC markers including α‐smooth muscle actin, desmin, vinculin and calponin in RT‐PCR , WB and ICC experiments. The survival rate of cultures on the hydrogel‐coated matrices was significantly higher than uncoated cultures.Conclusions Human EnSCs were successfully differentiated into SMCs , using hydrogels as scaffold. EnSCs may be used for autologous bladder wall regeneration without any immunological complications in women. Currently work is in progress using bioabsorbable nanocomposite materials as EnSC scaffolds for developing urinary bladder wall tissue.