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Distribution of phosphodiesterase type 5 ( PDE 5) in the lateral wall of the guinea pig urinary bladder
Author(s) -
Rahnama'i Mohammad S.,
Koeveringe Gommert A.,
Hohnen Ramona,
Ona Samsya,
Kerrebroeck Philip E.V.,
Wachter Stefan G.G.
Publication year - 2013
Publication title -
bju international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.773
H-Index - 148
eISSN - 1464-410X
pISSN - 1464-4096
DOI - 10.1111/bju.12031
Subject(s) - urothelium , cgmp specific phosphodiesterase type 5 , chemistry , calcitonin gene related peptide , urinary bladder , nitric oxide , seminal vesicle , endocrinology , medicine , biology , biochemistry , prostate , sildenafil , neuropeptide , receptor , cancer
Objective To study PDE 5 localisation by visualising the product of phosphodiesterase type 5 ( PDE 5) inhibition, namely cGMP , to determine the site of action of inhibitors in the urinary bladder.Materials and Methods Bladders of nine male guinea pigs were dissected and treated in wells containing 2 mL K rebs' solution and 1 μM of the specific PDE 5 inhibitor vardenafil at 36 ° C for 30 min. After stimulating tissues with 100 μM of the nitric oxide ( NO ) donor diethylamine‐ NONO ate for 10 min, the tissues were snap‐frozen and 9–10 μm sections were cut. Sections were examined for cGMP immunoreactivity and also stained for vimentin, a marker for interstitial cells and the neuromarkers protein gene product 9.5 ( PGP 9.5), synaptic vesicle protein 2 ( SV 2), neurofilament ( NF ) and calcitonin gene‐related peptide ( CGRP ), using the two‐step indirect immunohistochemistry technique.Results After PDE 5 inhibition, cGMP was found to be present in the urothelium, suburothelial interstitial cells and endothelium of blood vessels. cGMP was not expressed in nerves positive for CGRP , NF and SV 2, and was expressed only in very few efferent nerves positive for PGP 9.5.Conclusion Our data show that the possible sites of action of PDE 5 inhibition in the bladder are the urothelium, suburothelial interstitial cells and blood vessels, rather than the bladder nerve fibres.