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Flow cytometric analysis of cerebrospinal fluid improves detection of leukaemic blasts in infants with acute lymphoblastic leukaemia
Author(s) -
Thastrup Maria,
Marquart Hanne Vibeke,
Levinsen Mette,
Modvig Signe,
Abrahamsson Jonas,
Albertsen Birgitte Klug,
Frost BrittMarie,
HarilaSaari Arja,
Pesola Jouni,
Ulvmoen Aina,
Wojcik Dorota Malgorzata,
Taskinen Mervi,
Hoffmann Marianne,
Lausen Birgitte,
Schmiegelow Kjeld
Publication year - 2021
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/bjh.17769
Subject(s) - cerebrospinal fluid , flow cytometry , medicine , lumbar puncture , pathology , immunology
Summary Infants with acute lymphoblastic leukaemia (ALL) have a high frequency of central nervous system (CNS) involvement. Flow cytometric analysis of cerebrospinal fluid (CSF) was recently demonstrated to be a sensitive method for detecting CNS involvement in childhood ALL. In the present study, CSF from 14 infants was collected at routine lumbar punctures and analysed by multicolour flow cytometry. At initial diagnosis, leukaemic blasts were detected in CSF by flow cytometry in 11 patients (78·6%) compared to seven patients (50%) by cytospin. Larger studies are needed to determine if CSF flow cytometry has prognostic value in infant ALL.