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Variables affecting the presence of mesenchymal stromal cells in peripheral blood and their relationship with apheresis products
Author(s) -
Jain Ankur,
Khadwal Alka,
Sachdeva Man Updesh Singh,
Bose Praveen,
Lad Deepesh,
Bhattacharya Shalmoli,
Prakash Gaurav,
Malhotra Pankaj,
Varma Neelam,
Varma Subhash
Publication year - 2020
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/bjh.16412
Subject(s) - mesenchymal stem cell , apheresis , stromal cell , medicine , adipose tissue , bone marrow , flow cytometry , ex vivo , transplantation , cell therapy , regeneration (biology) , stem cell , immunology , pathology , in vivo , biology , microbiology and biotechnology , platelet
Summary Mesenchymal stromal cells (MSCs) demonstrate the properties of self‐renewal, multipotentiality, and immunosuppression, which are responsible for their widespread clinical applications in tissue repair and regeneration. MSCs have been isolated from bone marrow, adipose tissue, and cord blood using culture in specialised media. Their presence in peripheral blood (PB) is debatable. We studied the presence of MSCs at baseline (PB) and following mobilisation with growth factors [PB and apheresis product (AP)] in patients undergoing autologous stem cell transplantation and healthy donors using flow cytometry. We conclude that both mobilised PB and AP are potential sources of MSCs. Given their small numbers in PB/AP, clinical use is feasible following ex‐vivo expansion. Variables affecting the presence of MSCs in PB and AP are also discussed.