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Potential involvement of ubiquitin‐proteasome system dysfunction associated with oxidative stress in the pathogenesis of sickle cell disease
Author(s) -
Warang Prashant,
Homma Takujiro,
Pandya Riddhi,
Sawant Anuja,
Shinde Nikhil,
Pandey Debjeet,
Fujii Junichi,
Madkaikar Manisha,
Mukherjee Malay B.
Publication year - 2018
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/bjh.15437
Subject(s) - proteasome , pathogenesis , pathophysiology , ubiquitin , oxidative stress , disease , medicine , immunology , biology , endocrinology , biochemistry , gene
Summary The ubiquitin‐proteasome system ( UPS ) is an important intracellular proteolytic pathway responsible for the degradation of proteins and oxidative damage; hence it plays a central role in maintaining homeostasis of red blood cells ( RBC s). The present study investigated the levels of polyubiquitination, the function of proteasomes and effect of hydroxycarbamide ( HC ) therapy in RBC s from sickle cell disease ( SCD ) patients. Polyubiquitinated proteins were found to be elevated in untreated SCD ( UT ‐ SCD ) patients compared to those in HC ‐treated SCD patients ( HC ‐ SCD ) and controls. Activities of β1 and β2 subunits were a little higher in UT ‐ SCD patients, and much higher proteolytic activities were observed in all three subunits (β1, β2 and β5) of RBC s in HC ‐ SCD patients compared to those of UT ‐ SCD patients and controls, although the protein levels of these subunits remained approximately the same. It is notable that, despite HC therapy, some patients showed persistent complications and accumulation of polyubiquitinated proteins. The enhanced proteasomal activity among HC ‐treated patients might remove the polyubiquitinated protein and could be one of the important mechanisms of therapeutic action. These findings could be useful to understand the pathophysiology of SCD and its clinical heterogeneity and identify a suitable therapeutic target for the better management of these patients.