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Detection of platelet autoantibodies to identify immune thrombocytopenia: state of the art
Author(s) -
Porcelijn Leendert,
Huiskes Elly,
Oldert Gonda,
Schipperus Martin,
Zwaginga Jaap J.,
Haas Masja
Publication year - 2018
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/bjh.15404
Subject(s) - autoantibody , medicine , immune thrombocytopenia , platelet , immunology , antibody , monoclonal antibody , platelet membrane glycoprotein , immune system , antigen , monoclonal
Summary Immune Thrombocytopenia ( ITP ) is diagnosed by exclusion of other causes for thrombocytopenia. Reliable detection of platelet autoantibodies would support the clinical diagnosis of ITP and prevent misdiagnosis. We optimized our diagnostic algorithm for suspected ITP using the direct monoclonal antibody immobilization of platelet antigens assay ( MAIPA ), which evaluates the presence of platelet autoantibodies on the glycoproteins ( GP ) II b/ III a, Ib/ IX and V bound on the patient platelets. The direct MAIPA was shown to be a valuable technique for the detection of platelet autoantibodies and could possibly become a guide for optimizing therapy towards a more personalized treatment of ITP .