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Elevated levels of macromolecular damage are correlated with increased nitric oxide synthase expression in erythrocytes isolated from twin neonates
Author(s) -
Dugmonits Kriszti.,
Ferencz Ágnes,
Zahorán Szabolcs,
Lázár Renáta,
Talapka Petra,
Orvos Hajnalka,
Hermesz Edit
Publication year - 2016
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/bjh.14156
Subject(s) - oxidative stress , peroxynitrite , nitric oxide , reactive oxygen species , nitric oxide synthase , andrology , hydrogen peroxide , nitrosylation , biology , chemistry , medicine , endocrinology , biochemistry , superoxide , enzyme
Summary Pregnancy is a state associated with an enhanced metabolism and demand for O 2 , which may lead to the overproduction of reactive oxygen species ( ROS ) and hence to oxidative stress. An elevated ROS level may result in delayed development and a low birth weight. The aim of this study was to reveal the consequences of multiple pregnancies on the redox status of neonatal human red blood cells ( RBC s) and evaluate the role of endothelial nitric oxide synthase ( NOS 3) – expressing RBC s in the generation of oxidative stress. The study presents evidence of higher levels of production of hydrogen peroxide, peroxynitrite and nitrate content in the RBC s of twin neonates, clearly reflected by an elevated level of protein and lipid damages. This phenotype appears to be a consequence of multiple pregnancies, regardless of the level of maturity or the birth weight of the twins. Besides the higher level of ROS , there was a general decrease in the expression of genes coding for antioxidants. The first data are presented on NOS 3‐expressing neonatal human RBC s. The number of RBC s producing NOS 3 was more than twice as high in twin neonates compared to singletons, with no correlation to maturity.