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Cell cycle status in AML blast cells from peripheral blood, bone marrow aspirates and trephines and implications for biological studies and treatment
Author(s) -
Sellar Rob S.,
Fraser Laura,
Khwaja Asim,
Gale Rosemary E.,
Marafioti Teresa,
Akarca Ayse,
Hubank Mike,
Brooks Tony,
Stoeber Kai,
Williams Gareth,
Linch David C.
Publication year - 2016
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/bjh.14055
Subject(s) - bone marrow , peripheral blood , medicine , precursor cell , pathology , cell , immunology , biology , genetics
Using immunohistochemistry and flow cytometry to define phases of the cell cycle, this study shows that a high proportion of acute myeloid leukaemia ( AML ) blasts obtained from trephine biopsies are cycling, whereas >95% of peripheral blood‐derived blasts are arrested in G 1 . Results obtained from bone marrow aspirates are more similar to those from blood rather than from trephine biopsies. These differences were confirmed by gene expression profiling in a patient with high count AML . This has implications for cell cycle and other biological studies using aspirates rather than trephine biopsies and for the use of cell mobilising agents before chemotherapy.