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Characterization of bone marrow mesenchymal stromal cells in aplastic anaemia
Author(s) -
Hamzic Edita,
Whiting Karen,
Gordon Smith Edward,
Pettengell Ruth
Publication year - 2015
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/bjh.13364
Subject(s) - clonogenic assay , mesenchymal stem cell , stromal cell , haematopoiesis , bone marrow , stem cell , in vitro , cancer research , immunology , biology , microbiology and biotechnology , biochemistry
Summary In aplastic anaemia ( AA ), haemopoietic activity is significantly reduced and generally attributed to failure of haemopoietic stem cells ( HSC ) within the bone marrow ( BM ). The regulation of haemopoiesis depends on the interaction between HSC and various cells of the BM microenvironment, including mesenchymal stromal cells ( MSC ). MSC involvement in the functional restriction of HSC in AA is largely unknown and therefore, the physical and functional properties of AA MSC were studied in vitro . MSC were characterized by their phenotype and ability to form adherent stromal layers. The functional properties of AA MSC were assessed through proliferative, clonogenic and cross‐over culture assays. Results indicate that although AA MSC presented typical morphology and distinctive mesenchymal markers, stromal formation was reduced, with 50% of BM samples failing to produce adherent layers. Furthermore, their proliferative and clonogenic capacity was markedly decreased ( P = 0·03 and P = 0·04 respectively) and the ability to sustain haemopoiesis was significantly reduced, as assessed by total cell proliferation ( P = 0·032 and P = 0·019 at Week 5 and 6, respectively) and clonogenic potential of HSC ( P = 0·02 at Week 6). It was concluded that the biological characteristics of AA MSC are different from those of control MSC and their in vitro haemopoiesis‐supporting ability is significantly reduced.