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Reduced MIR 130 A is involved in primary immune thrombocytopenia via targeting TGFB 1 and IL 18
Author(s) -
Zhao Haifeng,
Li Huiyuan,
Du Weiting,
Zhang Donglei,
Ge Jing,
Xue Feng,
Zhou Zeping,
Yang Renchi
Publication year - 2014
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/bjh.12934
Subject(s) - microrna , pathogenesis , peripheral blood mononuclear cell , immunology , medicine , immune system , interleukin 18 , autoimmune disease , taqman , interleukin , real time polymerase chain reaction , gene expression , gene , biology , cytokine , antibody , genetics , in vitro
Summary Micro RNA s (mi RNA s) play a vital role in the regulation of immunological functions and prevention of autoimmune disease. The abnormal expressions of several mi RNA s in patients with the acquired autoimmune disease, immune thrombocytopenia ( ITP ), have been reported. However, the exact mechanism of mi RNA s in the pathogenesis of ITP is currently not well understood. This study examined the mi RNA expression profile of peripheral blood mononuclear cells ( PBMC s) in ITP patients by mi RNA array and T aq M an real‐time polymerase chain reaction. MIR 130 A expression was found to be significantly decreased in PBMC s from patients with active chronic ITP compared with that of normal controls. Subsequently, dual‐luciferase reporter gene analysis was used to validate that MIR 130A targeted the transforming growth factor‐beta1 ( TGFB 1 ) and interleukin 18 ( IL 18 ) genes. In addition, we also monitored the dynamic expression of MIR 130 A and its targeted genes pre‐ and post‐treatment of ITP patients and determined that the expression of MIR 130 A and TGFB 1 was up‐regulated, whereas IL 18 expression was down‐regulated after effective treatment. In conclusion, this study suggests that reduced MIR 130 A is involved in ITP via targeting of TGFB 1 and IL 18 expression.