Premium
Effects of oxidative stress on red blood cell rheology in sickle cell patients
Author(s) -
Hierso Régine,
Waltz Xavier,
Mora Pierre,
Romana Marc,
Lemonne Nathalie,
Connes Philippe,
HardyDessources MarieDominique
Publication year - 2014
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/bjh.12912
Subject(s) - oxidative stress , cell , medicine , rheology , red cell , red blood cell , sickle cell anemia , blood cell , immunology , chemistry , biochemistry , disease , materials science , composite material
Summary Sickle cell anaemia ( SS ) and sickle cell‐haemoglobin C disease ( SC ) patients exhibit severe red blood cell ( RBC ) rheological alterations involved in the development of several complications. The contribution of oxidative stress in these haemorheological abnormalities is still unknown. We compared RBC reactive oxygen species ( ROS ) and glutathione ( GSH ) content, and the haemorheological profile of SS ( n = 11), SC ( n = 11) and healthy subjects ( n = 12) at baseline and after in‐vitro treatment with t‐butyl hydroperoxide ( TBHP ). We showed: (i) higher RBC ROS content in SS and SC patients, with the highest level observed in SS patients; (ii) lower RBC GSH content in sickle syndrome patients, especially in SS patients; (iii) TBHP increased RBC ROS production and decreased RBC GSH content in all groups; (iv) TBHP decreased RBC aggregation and increased the strength of RBC aggregates in all groups but the increase in RBC aggregates strength was greater in sickle cell patients; (v) TBHP decreased RBC deformability in the three groups but with a higher magnitude in sickle cell patients. These data suggest that RBC s from sickle cell patients have an exaggerated response to oxidative stress, which is accompanied by a profound abnormal haemorheological profile, with greater alterations in SS than in SC patients.