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Flow cytometric identification of 76 patients with biclonal disease among 5523 patients with chronic lymphocytic leukaemia (B‐ CLL ) and its genetic characterization
Author(s) -
Kern Wolfgang,
Bacher Ulrike,
Schnittger Susanne,
Dicker Frank,
Alpermann Tamara,
Haferlach Torsten,
Haferlach Claudia
Publication year - 2014
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/bjh.12652
Subject(s) - ighv@ , flow cytometry , chronic lymphocytic leukemia , fluorescence in situ hybridization , monoclonal , medicine , hematology , cytogenetics , biology , chromosome , pathology , monoclonal antibody , immunology , genetics , gene , leukemia , antibody
Summary Multiparameter flow cytometry ( MFC ) identifies rare cases of biclonal disease in chronic lymphocytic leukaemia ( CLL ). By MFC , we identified 76 patients with biclonal disease in a cohort of 5523 CLL patients (1·4%). Fluorescence in situ hybridization and chromosome banding analysis revealed five and six cases, respectively, with two different cytogenetic aberrations due to clonal evolution. Two different B‐cell receptor rearrangements and IGHV subtypes were more frequent in biclonal than in monoclonal CLL by MFC (37·1% vs. 2·7%; P < 0·001). Patients with biclonal CLL by MFC showed a trend to a shorter time to treatment than monoclonal CLL ( P = 0·080).