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Flow cytometry thresholds of myeloperoxidase detection to discriminate between acute lymphoblastic or myeloblastic leukaemia
Author(s) -
Guy Julien,
AntonyDebré Iléana,
Benayoun Emmanuel,
Arnoux Isabelle,
Fossat Chantal,
GarffTavernier Magali,
Raimbault Anna,
Imbert Michèle,
Maynadié Marc,
Lacombe Francis,
Béné Marie C,
WagnerBallon Orianne
Publication year - 2013
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/bjh.12277
Subject(s) - myeloperoxidase , flow cytometry , myeloid , medicine , population , cytochemistry , immunology , myeloid leukaemia , pathology , inflammation , environmental health , ultrastructure
Summary The W orld H ealth O rganization 2008 Classification emphasizes myeloperoxidase ( MPO ) detection as sufficient for assigning a blast population to the myeloid lineage. Published MPO positivity thresholds are 10% for flow cytometry ( FCM ) but 3% for cytochemistry. Here we re‐evaluated the FCM ‐ MPO threshold by comparing retrospectively 128 acute lymphoblastic leukaemias and 75 acute myeloid leukaemias without maturation, all assessed by benzidine‐based cytochemistry. A 13% threshold was found to be relevant using an isotype control as background‐reference (sensitivity 95·1%, specificity 91·7%). Residual normal lymphocytes proved to be an advantageous alternative reference, a threshold of 28% yielding improved 97·4% sensitivity and 96·1% specificity.