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Reduced expression of MIR 409‐3p in primary immune thrombocytopenia
Author(s) -
Li Huiyuan,
Zhao Haifeng,
Xue Feng,
Zhang Xian,
Zhang Donglei,
Ge Jing,
Yang Yanhui,
Xuan Min,
Fu Rongfeng,
Yang Renchi
Publication year - 2013
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/bjh.12213
Subject(s) - microrna , peripheral blood mononuclear cell , immune system , immunology , pathogenesis , autoimmunity , taqman , biology , microarray , gene expression , gene , regulation of gene expression , immune thrombocytopenia , real time polymerase chain reaction , antibody , genetics , in vitro
Summary Primary immune thrombocytopenia ( ITP ) is an acquired autoimmune disease with many immune dysfunctions. Micro RNA s (mi RNA s) are a class of non‐coding RNA s that post‐transcriptionally regulate gene expression by messenger RNA degradation or translational repression. Accumulating evidence has demonstrated that mi RNA s play a vital role in the regulation of immunological functions and prevention of autoimmunity. However, whether mi RNA s are involved in the pathogenesis of ITP is still unknown. To illustrate the role of mi RNA s in ITP , the expression profile of mi RNA s from peripheral blood mononuclear cells ( PBMC s) in ITP patients was investigated by mi RNA microarray, and further validated by TaqMan real‐time polymerase chain reaction. MIR 409‐3p expression was decreased in PBMC s of active ITP patients, but this recovered after effective therapy. IFNG was identified and validated as one of the targeted genes of MIR 409‐3p by bioinformatic prediction and reporter gene analysis. In addition, we found DGCR 8 transcript was down‐regulated in ITP patients and positively correlated with MIR 409‐3p . Thus, in ITP patients, decreased DGCR 8 leads to down‐regulation of MIR 409‐3p , which in turn results in up‐regulation of IFNG ( IFN ‐γ).