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Next‐generation sequencing – feasibility and practicality in haematology
Author(s) -
Kohlmann Alexander,
Grossmann Vera,
Nadarajah Niroshan,
Haferlach Torsten
Publication year - 2013
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/bjh.12194
Subject(s) - dna sequencing , amplicon sequencing , risk stratification , computational biology , minimal residual disease , deep sequencing , personalized medicine , computer science , amplicon , medicine , medical physics , bioinformatics , biology , gene , genetics , polymerase chain reaction , genome , 16s ribosomal rna , leukemia
Summary Next‐generation sequencing platforms have evolved to provide an accurate and comprehensive means for the detection of molecular mutations in heterogeneous tumour specimens. Here, we review the feasibility and practicality of this novel laboratory technology. In particular, we focus on the utility of next‐generation sequencing technology in characterizing haematological neoplasms and the landmark findings in key haematological malignancies. We also discuss deep‐sequencing strategies to analyse the constantly increasing number of molecular markers applied for disease classification, patient stratification and individualized monitoring of minimal residual disease. Although many facets of this assay need to be taken into account, amplicon deep‐sequencing has already demonstrated a promising technical performance and is being continuously developed towards routine application in diagnostic laboratories so that an impact on clinical practice can be achieved.