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Reduction of hyaluronan and increased expression of HYBID (alias CEMIP and KIAA1199) correlate with clinical symptoms in photoaged skin
Author(s) -
Yoshida H.,
Nagaoka A.,
Komiya A.,
Aoki M.,
Nakamura S.,
Morikawa T.,
Ohtsuki R.,
Sayo T.,
Okada Y.,
Takahashi Y.
Publication year - 2018
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/bjd.16335
Subject(s) - dermis , papillary dermis , staining , skin biopsy , wrinkle , biopsy , hyaluronic acid , pathology , dermatology , chemistry , human skin , medicine , biology , anatomy , gerontology , genetics
Summary Background Hyaluronan ( HA ) metabolism in skin fibroblasts is mediated by HYBID (hyaluronan binding protein involved in hyaluronan depolymerization, alias CEMIP and KIAA 1199) and the HA synthases HAS 1 and HAS 2. However, photoageing‐dependent changes in HA and their molecular mechanisms, and the relationship between HA metabolism and clinical symptoms in photoaged skin remain elusive. Objectives We examined the amount, size and tissue distribution of HA and expression levels of HYBID , HAS 1 and HAS 2 in photoaged skin, and analysed their relationship with the degree of photoageing. Methods Photoageing‐dependent changes of HA were investigated by studying skin biopsies isolated from photoprotected and photoexposed areas of the same donors, and the relationships between HA and photoageing symptoms such as skin wrinkling and sagging were examined. Results Skin biopsy specimens showed that the amount and size of HA are decreased in photoexposed skin compared with photoprotected skin, and this was accompanied by increased expression of HYBID and decreased expression of HAS 1 and HAS 2 . Histologically, HA staining in the papillary dermis was decreased in photoexposed skin, showing reverse correlation with HYBID expression. HYBID expression in the photoexposed skin directly correlated with skin roughness and sagging parameters, and the reduced HA staining in the papillary dermis in the photoexposed skin positively correlated with these symptoms. Conclusions These data demonstrate that imbalance between HYBID ‐mediated HA degradation and HAS ‐mediated HA synthesis may contribute to enhanced HA catabolism in photoaged skin, and suggest that HYBID ‐mediated HA reduction in the papillary dermis is related to skin wrinkling and sagging of photoaged skin.