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Integrative analyses reveal biological pathways and key genes in psoriasis
Author(s) -
Dou J.,
Zhang L.,
Xie X.,
Ye L.,
Yang C.,
Wen L.,
Shen C.,
Zhu C.,
Zhao S.,
Zhu Z.,
Liang B.,
Wang Z.,
Li H.,
Fan X.,
Liu S.,
Yin X.,
Zheng X.,
Sun L.,
Yang S.,
Cui Y.,
Zhou F.,
Zhang X.
Publication year - 2017
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/bjd.15682
Subject(s) - psoriasis , gene , biology , gene expression profiling , gata3 , wnt5a , gene expression , genetics , biological pathway , computational biology , immunology , transcription factor , wnt signaling pathway
Summary Background Psoriasis is a complex disease influenced by both genetic and environmental factors with abnormal gene expression in lesional skin. However, no studies are available on genome‐scale gene expression of psoriatic lesions in the Chinese population. In addition, systematic studies on the biological pathways, pathogenicity and interaction networks of psoriasis‐related genes with abnormal expression profiles require further investigation. Objectives To further explore the associated pathways in psoriasis by functional analysis and to identify the key genes by gene pathogenicity analysis. Methods We performed RNA sequencing on 60 skin biopsy samples from patients with psoriasis and healthy controls to identify the primary differentially expressed genes in psoriatic lesional skin. We retrieved all reported psoriasis‐associated genes and performed integrative analyses covering gene expression profiling, pathway analysis, gene pathogenicities and protein–protein interaction networks. Results We found that internal and external stimuli may activate immunoinflammatory responses to promote the development of psoriasis. Pathways associated with infectious diseases and cancers were identified by functional and pathway analyses. The gene pathogenicity analysis revealed five key genes in psoriasis: PPARD , GATA 3 , TIMP 3 , WNT 5A and PTTG 1 . Conclusions Our analyses showed that genes contributed to the pathogenesis of psoriasis by activating risk pathways with components abnormality in expression. We identified five potentially pathogenic genes for psoriasis that may serve as important biomarkers for the diagnosis and treatment.