Premium
Cross‐reactivity between methylisothiazolinone, octylisothiazolinone and benzisothiazolinone using a modified local lymph node assay
Author(s) -
Schwensen J.F.,
Menné Bonefeld C.,
Zachariae C.,
Agerbeck C.,
Petersen T.H.,
Geisler C.,
Bollmann U.E.,
Bester K.,
Johansen J.D.
Publication year - 2017
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/bjd.14825
Subject(s) - local lymph node assay , lymph node , lymph , medicine , immunology , cd8 , sensitization , immune system , pathology , skin sensitization
Summary Background In the light of the exceptionally high rates of contact allergy to the preservative methylisothiazolinone ( MI ), information about cross‐reactivity between MI , octylisothiazolinone ( OIT ) and benzisothiazolinone ( BIT ) is needed. Objectives To study cross‐reactivity between MI and OIT , and between MI and BIT . Methods Immune responses to MI , OIT and BIT were studied in vehicle and MI ‐sensitized female CBA mice by a modified local lymph node assay. The inflammatory response was measured by ear thickness, cell proliferation of CD 4 + and CD 8 + T cells, and CD 19 + B cells in the auricular draining lymph nodes. Results MI induced significant, strong, concentration‐dependent immune responses in the draining lymph nodes following a sensitization phase of three consecutive days. Groups of MI ‐sensitized mice were challenged on day 23 with 0·4% MI , 0·7% OIT and 1·9% BIT – concentrations corresponding to their individual EC 3 values. No statistically significant difference in proliferation of CD 4 + and CD 8 + T cells was observed between mice challenged with MI compared with mice challenged with BIT and OIT . Conclusions The data indicate cross‐reactivity between MI , OIT and BIT , when the potency of the chemical was taken into account in choice of challenge concentration. This means that MI ‐sensitized individuals may react to OIT and BIT if exposed to sufficient concentrations.