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Temperature‐dependent impact of thermal aminolaevulinic acid photodynamic therapy on apoptosis and reactive oxygen species generation in human dermal fibroblasts
Author(s) -
Mamalis A.,
Koo E.,
Sckisel G.D.,
Siegel D.M.,
Jagdeo J.
Publication year - 2016
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/bjd.14509
Subject(s) - photodynamic therapy , reactive oxygen species , superoxide , apoptosis , programmed cell death , chemistry , radical , cancer research , in vivo , fibroblast , in vitro , medicine , biology , biochemistry , enzyme , microbiology and biotechnology , organic chemistry
Summary Background Actinic keratoses ( AK s) are generally accepted as common precursor lesions to invasive squamous cell carcinoma. Photodynamic therapy ( PDT ) is a common, in‐office, field therapy modality used in the treatment of AK s. Clinical and laboratory observations have demonstrated that temperature modulation can affect PDT efficacy. Objectives To demonstrate thermal PDT increases apoptotic cell death, and to investigate the mechanistic role of reactive oxygen species ( ROS ) free radicals in an in vitro human skin fibroblast model. Methods This study was completed using commercially available primary human skin fibroblasts treated with aminolaevulinic acid ( ALA ) at specific concentrations and controlled temperatures. Cell death, apoptosis and superoxide ROS levels were quantified. Results We found that thermal PDT with 0·5 mmol L −1 ALA resulted in significant temperature‐dependent increases in total apoptosis and superoxide ROS generation between 33 °C and 42 °C. Conclusions Our results indicate that thermal PDT significantly increases apoptotic cell death through increased generation of superoxide ROS in a temperature‐dependent manner.