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Impact of oncogenic BRAF mutations and p16 expression on the growth rate of early melanomas and naevi in vivo
Author(s) -
Tschandl P.,
Berghoff A.S.,
Preusser M.,
Pammer J.,
Pehamberger H.,
Kittler H.
Publication year - 2016
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/bjd.14323
Subject(s) - in vivo , melanoma , mutation rate , dermatoscopy , mutation , cancer research , doubling time , biology , nevus , medicine , pathology , cell , genetics , gene
Summary Background It is important to know what drives and arrests melanocytic growth in vivo but observations linking oncogenic mutations to growth rates of melanocytic neoplasms in vivo are sparse. Objectives To clarify the relationship between BRAF V 600E mutations and p16 expression and the growth rate of melanocytic neoplasms in vivo . Methods We measured the growth rate of 54 melanocytic lesions (26 melanomas, 28 naevi) in vivo with digital dermatoscopy and correlated it with BRAF V 600E and p16 expression, and with dermatoscopic and histological patterns. Results Melanomas grew faster than naevi (mean 2·7 vs. 0·8 mm 2 /year; P < 0·001) and the growth rate was faster in lesions with more nests (> 25% nests: 2·0 mm 2 /year vs. < 25% nests: 1·0 mm 2 /year; P = 0·036). Melanomas with the BRAF V 600E mutation grew significantly faster than melanomas without the mutation (mean 3·36 vs. 1·60 mm 2 /year, P = 0·018). This effect of the BRAF V 600E mutation on the growth rate was not observed in melanocytic naevi (mean 1·01 vs. 0·47 mm 2 /year, P = 0·274). Histopathologically, extensive nesting, larger nests and larger cell sizes were more common in melanocytic neoplasms with the BRAF V 600E mutation than in those without the mutation. Melanomas expressing p16 had a slower growth rate than melanomas without p16 expression (2·27 vs. 4·34 mm 2 /year, P = 0·047). This effect was not observed in naevi (0·81 vs. 0·68 mm 2 /year, P = 0·836). Conclusions The expression of BRAF V 600E and the loss of p16 accelerate the growth rate of early melanomas in vivo but not in melanocytic naevi. In comparison to melanocytic proliferations that lack the mutation, the epidermal melanocytes in lesions that harbour BRAF V 600E mutations are larger and more frequently arranged in large nests.