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A functional single‐nucleotide polymorphism in the ERCC 1 gene alters the efficacy of narrowband ultraviolet B therapy in patients with active vitiligo in a Chinese population
Author(s) -
Dai W.,
Zhou F.B.,
Wei C.,
Wang X.W.,
Guo S.,
Yi X.L.,
Li K.,
Gao T.W.,
Liu L.,
Li C.Y.
Publication year - 2015
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/bjd.13892
Subject(s) - ercc1 , nucleotide excision repair , vitiligo , single nucleotide polymorphism , dna repair , pyrimidine dimer , genotype , cancer research , biology , medicine , microbiology and biotechnology , gene , immunology , genetics
Summary Background T lymphocytes have been shown to cause the destruction of melanocytes in vitiligo pathogenesis. Narrowband ultraviolet B ( NB ‐ UVB ), as an effective therapeutic strategy in vitiligo, can lead to the formation of DNA photoproducts such as cyclobutane pyrimidine dimers ( CPD s) in perilesional lymphocytes and thus induce skin immunosuppression. The repair of DNA photoproducts is performed mainly through the nucleotide excision repair ( NER ) pathway. We hypothesized that single‐nucleotide polymorphisms ( SNP s) in NER genes might influence the repair capacity of CPD s and thus contribute to variations in phototherapy efficiency. Objectives To detect genetic polymorphisms in NER genes and their relationship with the efficacy of NB ‐ UVB therapy in patients with active vitiligo. Methods We investigated the association of NER SNP s ( XPA A23G, XPC Ci11A, XPC C2919A and ERCC 1 C118T) with phototherapy efficacy in 86 patients with vitiligo who received NB ‐ UVB treatment. Furthermore, we examined the impact of ERCC 1 C118T on the apoptosis of T lymphocytes and CPD accumulation after NB ‐ UVB irradiation. Results We found that patients with vitiligo with the ERCC 1 codon 118 CC genotype showed better efficacy after NB ‐ UVB irradiation than those with the ERCC 1 118 TT and CT genotypes, whereas no such association was documented among the genotypes of XPA A23G, XPC Ci11A or XPC C2919A. Additionally, the apoptosis rates and CPD levels of lymphocytes after NB ‐ UVB irradiation in patients with the ERCC 1 118 CC genotype were significantly higher than those in patients with the ERCC 1 118 TT and CT genotypes. Conclusions The ERCC 1 118 CC genotype confers better efficacy of NB ‐ UVB therapy in patients with active vitiligo.