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Inhibitory and anti‐inflammatory effects of the Helicobacter pylori ‐derived antimicrobial peptide HPA 3 NT 3 against Propionibacterium acnes in the skin
Author(s) -
Ryu S.,
Park Y.,
Kim B.,
Cho S.M.,
Lee J.,
Lee H.H.,
Gurley C.,
Song K.,
Johnson A.,
Armstrong C.A.,
Song P.I.
Publication year - 2014
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/bjd.13480
Subject(s) - propionibacterium acnes , in vivo , microbiology and biotechnology , inflammation , antimicrobial , antimicrobial peptides , cathelicidin , in vitro , acne , biology , chemistry , immunology , biochemistry , genetics
Summary Background An effective treatment strategy for acne vulgaris is the reduction of Propionibacterium acnes in the skin. The Helicobacter pylori ‐derived synthetic antimicrobial peptide HPA 3 NT 3 is a customized α‐helical cationic peptide with antibacterial and anti‐inflammatory activity. Objectives To examine the role of HPA 3 NT 3 as a treatment against P. acnes ‐induced skin inflammation. Methods Morphological alteration of individual P. acnes cells by HPA 3 NT 3 was visualized by scanning electron microscopy. Modulation by HPA 3 NT 3 of a number of P. acnes ‐induced innate immune responses was analysed in vitro using cultured normal human keratinocytes ( HK s), and in vivo using the ICR mouse, a well‐established model for P. acnes ‐induced skin inflammation. Results The minimum inhibitory concentration of HPA 3 NT 3 against P. acnes was low (0·4 μmol L −1 ). HPA 3 NT 3 showed no cytotoxicity to HK cells at the concentrations used in our in vitro and in vivo studies. Treatment with HPA 3 NT 3 in vitro induced morphological disruptions in P. acnes cells suggestive of a bactericidal effect. HPA 3 NT 3 significantly decreased P. acnes ‐induced interleukin‐8 expression and intracellular calcium mobilization in HK cells by inhibiting P. acnes ‐activated Toll‐like receptor 2‐mediated nuclear factor‐κB signalling pathways. Intradermal injection of HPA 3 NT 3 in vivo effectively decreased viable P. acnes , as well as erythema, swelling and inflammatory‐cell infiltration in ICR mouse ears inoculated with P. acnes . Conclusions Our data suggest that HPA 3 NT 3 has potential as a therapeutic agent for acne vulgaris due to its antimicrobial effects on P. acnes and its ability to block P. acnes ‐induced inflammation.