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Intravenous transplantation of bone marrow mesenchymal stem cells could effectively promote vascularization and skin regeneration in mechanically stretched skin
Author(s) -
Zhou S.B.,
Chiang C.A.,
Liu K.,
Li Q.F.
Publication year - 2015
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/bjd.13251
Subject(s) - ninth , medicine , china , transplantation , chiang mai , reconstructive surgery , shanghai china , surgery , general surgery , history , sociology , ethnology , physics , archaeology , regional science , acoustics
Summary Background Skin expansion is a procedure that stimulates and promotes skin regeneration by applying continuous mechanical stretching. However, the outcome of treatment is limited by the skin's instinctive regeneration capacity. Objectives To evaluate the impact of intravenous transplantation of bone marrow mesenchymal stem cells ( MSC s) on expanded skin regeneration. Methods MSC s from luciferase‐Tg Lewis rats were transplanted into a rat tissue expansion model and tracked in vivo by luminescence imaging. At the end of 21 days of skin expansion, the expanded skin was harvested and skin regeneration was evaluated by inflation volume, skin area and thickness. Counting of capillaries and vascular endothelial cell growth factor ( VEGF ) expression analysis were conducted to assess the impact of MSC s on expanded skin vascularization. Results Samples of the skin expansion model from the MSC group were observed to have a significantly higher inflation volume and greater expanded skin area than those from the control group at the end of 21 days’ follow‐up. In vivo tracing results showed that MSC s were recruited by mechanical stretch and migrated to expanded skin. Migrated MSC s promoted skin vascularization by secreting VEGF and differentiating into CD 31+ endothelial cells. Skin sections from the MSC group had a significant advantage in thickness and proliferating cell count, indicating that MSC s effectively enhanced expanded skin regeneration. Conclusions Intravenous transplantation of MSC s could effectively promote expanded skin regeneration. Transplanted MSC s could be recruited by mechanical stretch and subsequent migration to expanded skin. Engrafted MSC s could contribute to vascularization and cell proliferation.