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Detection of human papillomavirus ( HPV ) in patients with squamous cell carcinoma and the clinical characteristics of HPV ‐positive cases
Author(s) -
Shimizu A.,
Kato M.,
Takeuchi Y.,
Sano T.,
Kaira K.,
Uezato H.,
Ishikawa O.
Publication year - 2014
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/bjd.13234
Subject(s) - human papillomavirus , hpv infection , polymerase chain reaction , immunohistochemistry , pathology , in situ hybridization , papillomaviridae , medicine , sex organ , typing , lesion , biology , gene , cancer , messenger rna , cervical cancer , biochemistry , genetics
Summary Background The association between human papillomavirus ( HPV ) and squamous cell carcinoma ( SCC ) has been reported; however, the prevalence of HPV infection varies, and the clinical characteristics of HPV ‐positive cases remain unknown. Objectives To elucidate the frequency of HPV infection in a series of Japanese patients with SCC and to identify the characteristics of HPV ‐positive cases. Methods We evaluated 38 patients with SCC treated at our department. HPV typing was performed using SCC samples from different body sites. Immunohistochemical staining for HPV proteins and p16 INK 4a was performed, in addition to polymerase chain reaction and in situ hybridization. The clinical characteristics of the HPV ‐positive cases were clarified. Results Two genital lesions were positive for HPV type 16. Both cases showed basaloid features histopathologically, and were considered to have SCC that had arisen from bowenoid papulosis. p16 INK 4a expression was observed in 11 cases, including the two HPV ‐positive cases. Conclusions The present study indicates that the prevalence of HPV is not high and that bowenoid papulosis is an HPV ‐associated precancerous lesion. Further investigation is necessary to assess the relationship between HPV infection and SCC .