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Increased number and frequency of group 3 innate lymphoid cells in nonlesional psoriatic skin
Author(s) -
DyringAndersen B.,
Geisler C.,
Agerbeck C.,
Lauritsen J.P.H.,
Gúdjonsdottir S.D.,
Skov L.,
Bonefeld C.M.
Publication year - 2014
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/bjd.12658
Subject(s) - psoriasis , innate lymphoid cell , immunology , nkg2d , pathogenesis , medicine , interleukin 22 , allergy , cytokine , human skin , interleukin 23 , immune system , interleukin 17 , interleukin , innate immune system , biology , cytotoxic t cell , biochemistry , in vitro , genetics
Summary Background Psoriasis is a common immune‐mediated inflammatory disease that affects the skin and joints. The interleukin ( IL )‐23/ IL ‐17 A axis and IL ‐22 play key roles in the pathogenesis of psoriasis. IL ‐23‐responsive innate lymphoid cells ( ILC s) with a high capacity to produce IL ‐17 and/or IL ‐22 have recently been identified and associated with inflammatory bowel diseases. The occurrence and role of ILC s in human skin are poorly understood. Objectives To describe the prevalence of the different ILC subpopulations in skin from healthy controls and patients with psoriasis or allergy to nickel. Methods Skin biopsies were taken from healthy skin, nonlesional and lesional psoriatic skin, and nickel‐ and petrolatum‐exposed skin from patients with contact allergy to nickel, and lymphocytes were isolated. The cells were stained and characterized by flow cytometry. Cytokine and ligand mRNA expression were measured by quantitative polymerase chain reaction. Results We found that members of the three groups of ILC s were present in human skin. Remarkably, the number and frequency of ROR γt + CD 56 + ILC 3s, which are known to produce IL ‐22, were elevated in both nonlesional and lesional skin from patients with psoriasis compared with healthy skin and skin from patients with contact allergy to nickel. Furthermore, skin ILC s expressed high levels of the natural killer receptor NKG 2 D . NKG 2 D binds to stress‐induced ligands, including major histocompatibility complex class I‐related chain A, which we found to be strongly upregulated in lesional skin from patients with psoriasis. Conclusion These results show that ILC s are present in human skin and indicate that ROR γt + CD 56 + ILC 3 may be involved in the pathogenesis of psoriasis.