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Detection of HLA‐B*58:01, the susceptible allele for allopurinol‐induced hypersensitivity, by loop‐mediated isothermal amplification
Author(s) -
Kwok J.,
Kwong K.M.
Publication year - 2013
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/bjd.12097
Subject(s) - allopurinol , allele , delayed hypersensitivity , human leukocyte antigen , medicine , immunology , genetics , biology , antigen , gene
Summary Background Allopurinol, a common medication for gout treatment, can cause rare but life‐threatening severe cutaneous adverse reactions. A strong pharmacogenetic association of human leucocyte antigen (HLA)‐B*58:01 with allopurinol‐induced drug hypersensitivity has been reported, especially in the Han Chinese population. Objectives To develop a rapid and simple loop‐mediated isothermal amplification (LAMP) assay of HLA‐B*58:01 and evaluate its feasibility in predicting allopurinol‐induced drug hypersensitivity. Methods Two sets of LAMP primers targeting exons 2 and 3 of HLA‐B*58:01 were designed. DNA extracted from 20 clinical blood samples of patients with gout was used to evaluate the effectiveness of the two LAMP primer sets for the detection of HLA‐B*58:01. Results The results were compared with routine clinical genotyping methods. All extracted DNA samples tested with the HLA‐B*58:01 LAMP assay showed agreement with the routine genotyping results. No amplifications were observed when unextracted blood samples were tested. Conclusions The HLA‐B*58:01 LAMP assay was confirmed to be simple, rapid and specific for the detection of HLA‐B*58:01, and therefore of potential value in the diagnosis of allopurinol‐induced hypersensitivity.