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Cytotoxic, Antiproliferative and Apoptosis‐inducing Activity of L‐Amino Acid Oxidase from Malaysian Calloselasma rhodostoma on Human Colon Cancer Cells
Author(s) -
Zainal Abidin Syafiq Asnawi,
Rajadurai Pathmanathan,
Chowdhury Md. Ezharul Hoque,
Ahmad Rusmili Muhammad Rusdi,
Othman Iekhsan,
Naidu Rakesh
Publication year - 2018
Publication title -
basic and clinical pharmacology and toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.805
H-Index - 90
eISSN - 1742-7843
pISSN - 1742-7835
DOI - 10.1111/bcpt.13060
Subject(s) - cytotoxic t cell , apoptosis , cytotoxicity , chemistry , cancer cell , microbiology and biotechnology , cancer , biochemistry , biology , in vitro , genetics
The aim of this study was to investigate the cytotoxic, antiproliferative activity and the induction of apoptosis by L‐amino acid oxidase isolated from Calloselasma rhodostoma crude venom ( CR ‐ LAAO ) on human colon cancer cells. CR ‐ LAAO was purified using three chromatographic steps: molecular exclusion using G‐50 gel filtration resin, ion‐exchange by MonoQ column and desalted on a G25 column. The purity and identity of the isolated CR ‐ LAAO was confirmed by SDS ‐ PAGE and LC ‐ MS / MS . CR ‐ LAAO demonstrated time‐ and dose‐dependent cytotoxic activity on SW 480 (primary human colon cancer cells) and SW 620 (metastatic human colon cancer cells) with an EC 50 values of 6 μg/ml and 7 μg/ml at 48 hr, respectively. Quantification of apoptotic cells based on morphological features demonstrated significant increase in apoptotic cell population in both SW 480 and SW 620 cells which peaked at 48 hr. Significant increase in caspase‐3 activity and reduction in Bcl‐2 levels were demonstrated following CR ‐ LAAO treatment. These data provide evidence on the potential anticancer activity of CR ‐ LAAO from the venom of C. rhodostoma for therapeutic intervention of human colon cancer.