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Combination of RIZ 1 Overexpression and Radiotherapy Contributes to Apoptosis and DNA Damage of HeLa and SiHa Cervical Cancer Cells
Author(s) -
Yang Shanshan,
Xing Linan,
Gu Lina,
Cheng Haiyan,
Feng Yue,
Zhang Yunyan
Publication year - 2018
Publication title -
basic and clinical pharmacology and toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.805
H-Index - 90
eISSN - 1742-7843
pISSN - 1742-7835
DOI - 10.1111/bcpt.13008
Subject(s) - hela , radioresistance , clonogenic assay , apoptosis , cancer research , cervical cancer , radiosensitivity , cancer , cancer cell , transfection , ionizing radiation , dna damage , radiation therapy , dna repair , biology , medicine , microbiology and biotechnology , cell culture , dna , irradiation , genetics , physics , nuclear physics
Although radiotherapy has been widely applied to treating cervical cancer in the clinic, its therapeutic efficacy is often restricted to the radioresistance of cancer cells. Retinoblastoma protein‐interacting zinc finger gene 1 ( RIZ 1) has been suggested as a tumour suppressor gene, whereas its role in cervical cancer with or without radiotherapy has been unclear. In this study, two cervical cancer cell lines, HeLa and SiHa cells, stably transfected with RIZ 1 overexpression plasmid were subjected to ionizing radiation, and their survival fractions were calculated by assessing their clonogenic abilities. Our results showed that the forced overexpression of RIZ 1 significantly reduced the clonogenic survival rates of both HeLa and SiHa cells exposed to ionizing radiation. By analysing the cell apoptotic status, we found that the RIZ 1‐overexpressed cervical cancer cells under ionizing radiation were more vulnerable to damage, and more γ‐H2 AX foci were found in these cells. Furthermore, the volumes of tumour xenografts formed by the RIZ 1‐overexpressed cells in nude mice under ionizing radiation were smaller than those generated by the control cells. There were more morphological changes, apoptosis cells and lower expression of PCNA in RIZ 1‐overexpressed tumour tissues of mice after exposure to ionizing radiation. Taken together, our study demonstrates that the overexpression of RIZ 1 combined with radiotherapy facilitates apoptosis and DNA damage of cervical cancer cells.