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Evolution of Novel 3D Culture Systems for Studies of Human Liver Function and Assessments of the Hepatotoxicity of Drugs and Drug Candidates
Author(s) -
Andersson Tommy B.
Publication year - 2017
Publication title -
basic and clinical pharmacology and toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.805
H-Index - 90
eISSN - 1742-7843
pISSN - 1742-7835
DOI - 10.1111/bcpt.12804
Subject(s) - drug , transcriptome , in vivo , hepatocyte , in vitro , spheroid , drug development , pharmacology , function (biology) , liver function , computational biology , human liver , biology , 3d cell culture , proteome , liver metabolism , cell culture , clinical trial , liver injury , medicine , microbiology and biotechnology , bioinformatics , biochemistry , gene , gene expression , genetics
The pharmaceutical industry urgently needs reliable pre‐clinical models to evaluate the efficacy and safety of new chemical entities before they enter the clinical trials. Development of in vitro model systems that emulate the functions of the human liver organ has been an elusive task. Cell lines exhibit a low drug‐metabolizing capacity and primary liver cells rapidly dedifferentiate in culture, which restrict their usefulness substantially. Recently, the development of hepatocyte spheroid cultures has shown promising results. The proteome and transcriptome in the spheroids were similar to the liver tissue, and hepatotoxicity of selected substances was detected at in vivo ‐relevant concentrations.