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Human BJ Fibroblasts is an Alternative to Mouse BALB /c 3T3 Cells in In Vitro Neutral Red Uptake Assay
Author(s) -
Mannerström Marika,
Toimela Tarja,
Sarkanen JerttaRiina,
Hein Tuula
Publication year - 2017
Publication title -
basic and clinical pharmacology and toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.805
H-Index - 90
eISSN - 1742-7843
pISSN - 1742-7835
DOI - 10.1111/bcpt.12790
Subject(s) - neutral red , cytotoxicity , 3t3 cells , in vitro , fibroblast , microbiology and biotechnology , toxicity , biology , chemistry , cell culture , biochemistry , toxicology , genetics , transfection , organic chemistry
Abstract The OECD GD 129 BALB /c 3T3 neutral red uptake ( NRU ) assay is a standardized test method for estimating starting dose for an acute oral systemic toxicity test in rodents. Mouse BALB /c 3T3 fibroblasts are the most commonly used cells in the NRU assay. We have previously transferred and validated BALB /c 3T3 NRU assay in our GLP laboratory. Subsequently, in order to obtain more human‐relevant cytotoxicity data, we performed an intralaboratory validation using human BJ fibroblasts in the NRU assay instead of mouse BALB /c 3T3 fibroblasts. Here, we present comparative cytotoxicity data of 26 different test chemicals (pharmaceuticals, industrial chemicals, pesticides and food additives) produced with both BALB /c 3T3 NRU and BJ NRU assays.