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Biochemical and Histological Effects of Thiamine Pyrophosphate against Acetaminophen‐Induced Hepatotoxicity
Author(s) -
Uysal Hilal Bektas,
Dağlı Bekir,
Yılmaz Mustafa,
Kahyaoğlu Fadime,
Gökçimen Alparslan,
Ömürlü İmran Kurt,
Demirci Buket
Publication year - 2016
Publication title -
basic and clinical pharmacology and toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.805
H-Index - 90
eISSN - 1742-7843
pISSN - 1742-7835
DOI - 10.1111/bcpt.12496
Subject(s) - thiamine pyrophosphate , malondialdehyde , glutathione , acetaminophen , oxidative stress , chemistry , pharmacology , pyrophosphate , antioxidant , thiamine , medicine , biochemistry , endocrinology , enzyme , cofactor
Abstract The aim of this study was to investigate whether thiamine pyrophosphate ( TPP ) has biochemical and histological preventive effects on oxidative liver damage induced by paracetamol ( APAP ). Rats were divided into the following groups: healthy control ( HG ), APAP ( AG , 1500 mg/kg, orally), thiamine pyrophosphate ( TPPG , 100 mg/kg, intraperitoneally), APAP + NAC ( ANAC , 100 mg/kg, intraperitoneally), APAP + TPP ( ATPG ) and APAP + NAC + TPP ( ANTG ). Oxidant, antioxidant parameters, liver function tests and histological assessment were performed between groups. Malondialdehyde levels in the AG , HG , TPPG , ANAC , ATPG and ANTG groups were 0.470 ± 0.210, 0.213 ± 0.004, 0.194 ± 0.001, 0.197 ± 0.06, 0.199 ± 0.008 and 0.173 ± 0.010 μmol/g protein, respectively. Total glutathione levels were 7.787 ± 0.395, 14.925 ± 0.932, 13.200 ± 0.984, 13.162 ± 0.486, 13.287 ± 0.787 and 13.500 ± 0.891 μ m /g protein, respectively. In the AG group, marked liver damage occurred with the elevation of liver function tests and oxidative stress markers, such as malondialdehyde, myeloperoxidase and nitric oxide ( p < 0.05). Biochemical results were congruent with the histological changes of oxidative damage. Compared to the AG group ( p < 0.05), TPP significantly reduced oxidant parameter levels in the ATPG group and simultaneously increased the antioxidant parameter levels of catalase and glutathione. The histological changes were improved to almost normal hepatic structure. Moreover, TPP had nearly the same hepatoprotective effect as NAC , and there was statistically no additional benefit with NAC co‐treatment. There was no statistically significant difference ( p > 0.05) among the ANAC , ANTG and ATPG groups in terms of oxidant/antioxidant levels. TPP proved to be as efficacious as standard therapy and may be beneficial in APAP ‐induced hepatotoxicity.