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Antitumour Activity of the Microencapsulation of Annona vepretorum Essential Oil
Author(s) -
Bomfim Larissa M.,
Menezes Leociley R. A.,
Rodrigues Ana Carolina B. C.,
Dias Rosane B.,
Gurgel Rocha Clarissa A.,
Soares Milena B. P.,
Neto Albertino F. S.,
Nascimento Magaly P.,
Campos Adriana F.,
Silva Lidércia C. R. C. e.,
Costa Emmanoel V.,
Bezerra Daniel P.
Publication year - 2016
Publication title -
basic and clinical pharmacology and toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.805
H-Index - 90
eISSN - 1742-7843
pISSN - 1742-7835
DOI - 10.1111/bcpt.12488
Subject(s) - essential oil , annonaceae , in vivo , chemistry , annona , cytotoxicity , in vitro , food science , traditional medicine , biology , botany , biochemistry , medicine , microbiology and biotechnology
Abstract Annona vepretorum Mart. (Annonaceae), popularly known as ‘bruteira’, has nutritional and medicinal uses. This study investigated the chemical composition and antitumour potential of the essential oil of A. vepretorum leaf alone and complexed with β‐cyclodextrin in a microencapsulation. The essential oil was obtained by hydrodistillation using a Clevenger‐type apparatus and analysed using GC ‐ MS and GC ‐ FID . In vitro cytotoxicity of the essential oil and some of its major constituents in tumour cell lines from different histotypes was evaluated using the alamar blue assay. Furthermore, the in vivo efficacy of essential oil was demonstrated in mice inoculated with B16‐F10 mouse melanoma. The essential oil included bicyclogermacrene (35.71%), spathulenol (18.89%), ( E )‐β‐ocimene (12.46%), α‐phellandrene (8.08%), o ‐cymene (6.24%), germacrene D (3.27%) and α‐pinene (2.18%) as major constituents. The essential oil and spathulenol exhibited promising cytotoxicity. In vivo tumour growth was inhibited by the treatment with the essential oil (inhibition of 34.46%). Importantly, microencapsulation of the essential oil increased in vivo tumour growth inhibition (inhibition of 62.66%).