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Serum Metabolomic Profiling of Sulphur Mustard‐Exposed Individuals Using 1 HN uclear Magnetic Resonance Spectroscopy
Author(s) -
Zamani Zahra,
Ghanei Mostafa,
Panahi Yunus,
Arjmand Mohammad,
Sadeghi Sedigheh,
Mirkhani Fatemeh,
Parvin Shahram,
Salehi Maryam,
Sahebkar Amirhossein,
Vahabi Farideh
Publication year - 2016
Publication title -
basic and clinical pharmacology and toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.805
H-Index - 90
eISSN - 1742-7843
pISSN - 1742-7835
DOI - 10.1111/bcpt.12441
Subject(s) - metabolome , sulfur mustard , metabolomics , metabolism , metabolic pathway , mustard plant , pharmacology , chemistry , metabolite , biochemistry , clinical chemistry , physiology , medicine , toxicity , biology , chromatography , brassica , agronomy
Sulphur mustard is an alkylating agent that reacts with different cellular components, causing acute and delayed complications that may remain for decades after exposure. This study aimed to identify differentially expressed metabolites between mustard‐exposed individuals suffering from chronic complications compared with unexposed individuals as the control group. Serum samples were obtained from 15 mustard‐exposed individuals and 15 apparently healthy unexposed individuals. Metabolomic profiling was performed using 1 H nuclear magnetic resonance spectroscopy, and analyses were carried out using Chenomex and MATLAB softwares. Metabolites were identified using Human Metabolome Database, and the main metabolic pathways were identified using MetaboAnalyst software. Chemometric analysis of serum samples identified 11 differentially expressed metabolites between mustard‐exposed and unexposed groups. The main pathways that were influenced by sulphur mustard exposure were related to vitamin B 6 (down‐regulation), bile acid (up‐regulation) and tryptophan (down‐regulation) metabolism. Metabolism of vitamin B 6 , bile acids and tryptophan are the most severely impaired pathways in individuals suffering from chronic mustard‐induced complications. These findings may find implications in the monitoring of exposed patients and identification of new therapeutic approaches.