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Inhibition of DMBA ‐induced Oral Squamous Cells Carcinoma Growth by Brazilian Red Propolis in Rodent Model
Author(s) -
Ribeiro Danielle R.,
Alves Ângela Valéria F.,
Santos Esaú P.,
Padilha Francine F.,
Gomes Margarete Z.,
Rabelo Alessandra S.,
Cardoso Juliana C.,
Massarioli Adna Prado,
Alencar Severino Matias,
AlbuquerqueJúnior Ricardo Luiz C.
Publication year - 2015
Publication title -
basic and clinical pharmacology and toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.805
H-Index - 90
eISSN - 1742-7843
pISSN - 1742-7835
DOI - 10.1111/bcpt.12374
Subject(s) - dmba , formononetin , saline , daidzein , chemistry , pharmacology , oral administration , biochanin a , medicine , cancer , carcinogenesis , genistein
Abstract We investigated the effect of oral administration of hydroalcoholic extract of Brazilian red propolis ( HERP ) on DMBA ‐induced oral squamous cell carcinomas ( OSCC ) in rodents. The chemical components of the HERP were assessed by high‐performance liquid chromatography (HPLC). Carcinogenesis was topically induced in the lower lip of 25 rats using 9,10‐dimethyl‐1,2‐benzanthracene ( DMBA ); the tumour was treated with saline ( TUM 1) and Tween 80 ( TUM 2) as well as HERP at 10, 50 and 100 mg/kg ( HERP 10, HERP 50 and HERP 100, respectively) for 20 weeks. Topical application of saline and oral administration of 100 mg/kg HERP was used in five rats as a control group ( CTR ). After 26 weeks, the histological malignancy grading and immunohistochemical expression of Ki‐67 and p16 INK 4A were assessed in the tumours/tissue samples. The compounds identified were propyl gallate, daidzein, catechin, epicatechin, formononetin and biochanin A. Formononetin, daidzein and biochanin A showed concentration of 23.29, 0.38 and 0.67 mg/g of HERP , respectively. HERP at doses of 50 and 100 mg/kg inhibited 40% of OSCC growth and promoted a 3‐week delay in development of clinically detectable tumours. Epithelial dysplasia was observed in all samples with no clinical tumour, except in CTR . No significant difference in the immunoexpression of Ki‐67 and p16 INK 4A was observed between HERP ‐treated and saline/Tween 80‐treated groups ( p > 0.05). Our results suggest that HERP exerts chemopreventive activity on the progression of DMBA ‐induced epithelial dysplasia to OSCC in an experimental model of labial carcinogenesis; however, this effect is not associated with Ki‐67 and p16 INK 4A immunoexpression.