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Phytoestrogen α‐Zearalanol exerts Antiapoptotic Effects in Differentiated PC 12 Cells via Oestrogen Receptor α
Author(s) -
Dong Yi Long,
Wang Yan Mei,
Yang Hong Ju,
Yang Xiao Zhou,
Yu Hai Jing
Publication year - 2015
Publication title -
basic and clinical pharmacology and toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.805
H-Index - 90
eISSN - 1742-7843
pISSN - 1742-7835
DOI - 10.1111/bcpt.12296
Subject(s) - apoptosis , cytoprotection , antagonist , chemistry , receptor , microbiology and biotechnology , programmed cell death , pharmacology , biology , biochemistry
Our previous studies have demonstrated that phytoestrogen α‐zearalanol (α‐ ZAL ) possesses potential benefits in alleviating cell apoptotic death just like oestrogen. However, the underlying mechanism is not fully understood. This study was designed to test the hypothesis that the neuroprotective effect of α‐ ZAL is mediated by oestrogen receptor ( ER ) as α‐ ZAL owns the benzene ring structure may interact with ER . The present results showed a significant increase in apoptosis in differentiated PC 12 cells after a 24‐hr exposure to amyloid β‐peptide fragment 25‐35 (Aβ 25‐35 ), accompanied by decreasing of bcl‐2 expression and increasing bax expression, whereas a pre‐treatment with α‐ ZAL ameliorated these changes induced by Aβ 25‐35 . In addition, the α‐ ZAL ‐mediated cytoprotection was abrogated by ER α antagonist but not by ER β antagonist. In summary, these data suggest that α‐ ZAL intervenes against Aβ‐induced apoptosis via intersecting bcl‐2‐bax apoptotic pathway in an ER α‐sensitive manner.