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Epigallocatechin Gallate Promotes the Development of Mouse 2‐Cell Embryos In Vitro by Regulating Mitochondrial Activity and Expression of Genes Related to p53 Signalling Pathway
Author(s) -
Zhang Weiyu,
Lv Junjie,
Zhang Yanqin,
Jiang Yufei,
Chu Chenfeng,
Wang Shie
Publication year - 2014
Publication title -
basic and clinical pharmacology and toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.805
H-Index - 90
eISSN - 1742-7843
pISSN - 1742-7835
DOI - 10.1111/bcpt.12252
Subject(s) - microbiology and biotechnology , embryo , biology , cell cycle , cell , apoptosis , reactive oxygen species , cell growth , mitochondrion , gene , gene expression , cell cycle checkpoint , biochemistry
Preliminary studies have found that the epigallocatechin gallate ( EGCG ) at proper concentration could promote development of pre‐implantation mouse embryos in vitro . However, the underlying mechanisms have not been well understood. In this study, we collected 1‐cell embryos from Kunming ( KM ) mice, cultured them in M 16 medium or M 16 medium supplemented with 10 μg/mL EGCG and investigated the effects of EGCG on mitochondrial activity and reactive oxygen species ( ROS ) level of 2‐cell embryos. Furthermore, we explored expression differences of genes related to p53 signalling pathway in 2‐cell embryos using a PCR array. The results showed that ROS level and mitochondrial membrane potential were significantly lower in embryos cultured in the EGCG group than in the M 16 group ( p < 0.05), while the adenosine triphosphate content was slightly lower than in the M 16 group ( p > 0.05). PCR array test results showed that 18 genes were differentially expressed, among which eight genes involving cell growth, cell cycle regulation and m RNA transcription were up‐regulated and 10 genes involving apoptosis, cell cycle arrest and DNA repair were down‐regulated in the EGCG groups. It is concluded that EGCG could promote the development of 1‐cell embryos in vitro possibly due to its ability to scavenge ROS and regulate mitochondrial activity. In addition, EGCG could influence expression of genes related to p53 signalling pathway in 2‐cell embryos and promote cell cycle progression.