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Effect of Danofloxacin on Reactive Oxygen Species Production, Lipid Peroxidation and Antioxidant Enzyme Activities in Kidney Tubular Epithelial Cell Line, LLC ‐ PK 1
Author(s) -
Yu ChunHong,
Liu ZhaoYing,
Sun LeiSheng,
Li YuJuan,
Zhang DaSheng,
Pan RenTao,
Sun ZhiLiang
Publication year - 2013
Publication title -
basic and clinical pharmacology and toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.805
H-Index - 90
eISSN - 1742-7843
pISSN - 1742-7835
DOI - 10.1111/bcpt.12110
Subject(s) - lipid peroxidation , oxidative stress , danofloxacin , reactive oxygen species , superoxide dismutase , chemistry , antioxidant , glutathione peroxidase , biochemistry , catalase , glutathione , enzyme , enrofloxacin , ciprofloxacin , antibiotics
The purpose of this study was to investigate the possibility that oxidative stress was involved in danofloxacin‐induced toxicity in renal tubular cells epithelial cell line ( LLC ‐ PK 1). Confluent LLC ‐ PK 1 cells were incubated with various concentrations of danofloxacin. The extent of oxidative damage was assessed by measuring the reactive oxygen species ( ROS ) level, lipid peroxidation, cell apoptosis and antioxidative enzyme activities. Danofloxacin induced a concentration‐dependent increase in the ROS production, not even cytotoxic conditions. Similarly, danofloxacin caused an about 4 times increase in the level of thiobarbituric acid reactive substances at the concentration of 400 μM for 24 hr, but it did not induce cytotoxicity and apoptosis. Antioxidant enzymes activities, such as superoxide dismutase ( SOD ) and catalase ( CAT ), were increased after treatment with 100, 200 and 400 μM of danofloxacin for 24 hr. The activity of glutathione peroxidase ( GPX ) was significantly decreased in a concentration‐dependent manner. In addition, ROS production, lipid peroxidation and GPX decline were inhibited by additional glutathione and N‐acetyl cysteine. These data suggested that danofloxacin could not induce oxidative stress in LLC ‐ PK 1 cells at the concentration (≤400 μM) for 24 hr. The increase levels of ROS and lipid peroxidation could be partly abated by the increase activities of SOD and CAT .