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Soluble Adhesion Molecules Correlate with Surface Expression in an In Vitro Model of Endothelial Activation
Author(s) -
Kjærgaard Anders G.,
Dige Anders,
Krog Jan,
Tønnesen Else,
Wogensen Lise
Publication year - 2013
Publication title -
basic and clinical pharmacology and toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.805
H-Index - 90
eISSN - 1742-7843
pISSN - 1742-7835
DOI - 10.1111/bcpt.12091
Subject(s) - cd31 , endothelial activation , umbilical vein , e selectin , cell adhesion molecule , adhesion , flow cytometry , tumor necrosis factor alpha , in vitro , endothelial stem cell , inflammation , stimulation , microbiology and biotechnology , icam 1 , vcam 1 , immunology , chemistry , endothelium , cell adhesion , biology , biochemistry , endocrinology , organic chemistry
Endothelial activation is a pivotal event in the development and progression of inflammation. Central to endothelial activation is the up‐regulation of cellular adhesion molecules (CAMs) including E‐selectin (CD62E), ICAM‐1 (CD54), VCAM‐1 (CD106) and PECAM‐1 (CD31). These CAMs are also found in soluble forms ( sCAM s). In this in vitro study of endothelial activation, we examined whether the levels of sCAM s correlate with the endothelial surface expression of CAMs in a dose‐dependent and time‐dependent manner. Such a correlation would support the use of sCAM s as surrogate markers for endothelial activation in inflammatory conditions. Human umbilical vein endothelial cells (HUVEC) were cultured with various concentrations of TNF‐α for 8 hr and at a fixed concentration of TNF‐α for various durations. The levels of soluble and surface‐bound E‐selectin, ICAM‐1, VCAM‐1 and PECAM‐1 were quantified by flow cytometry. TNF‐α stimulation increased CAM and sCAM expression in a dose‐dependent and time‐dependent manner. There was a significant positive correlation between the levels of ICAM‐1 and sICAM ‐1 and between the levels of VCAM and sVCAM ‐1 in both the dose–response and time–response experiments. A positive correlation between the levels of E‐selectin and sE ‐selectin was observed in the time–response experiment. This study supports the use of sCAM s as potential biomarkers of endothelial activation. In particular, the use of sICAM ‐1, sVCAM ‐1 and sE ‐selectin seems promising.