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Relationships between flavin‐containing mono‐oxygenase 3 ( FMO3 ) genotype and trimethylaminuria phenotype in a J apanese population
Author(s) -
Shimizu Makiko,
Allerston Charles K.,
Shephard Elizabeth A.,
Yamazaki Hiroshi,
Phillips Ian R.
Publication year - 2014
Publication title -
british journal of clinical pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.216
H-Index - 146
eISSN - 1365-2125
pISSN - 0306-5251
DOI - 10.1111/bcp.12240
Subject(s) - trimethylamine , genotype , trimethylamine n oxide , haplotype , biology , allele , phenotype , genetics , excretion , compound heterozygosity , population , urinary system , gene , medicine , chemistry , endocrinology , biochemistry , environmental health
Aim The aim of this study was to investigate relationships between flavin‐containing mono‐oxygenase 3 ( FMO3 ) genotype and phenotype (conversion of odorous trimethylamine into non‐odorous trimethylamine N ‐oxide) in a large Japanese cohort suffering from trimethylaminuria. Methods Urinary excretion of trimethylamine and trimethylamine N ‐oxide was determined for 102 volunteers with self‐reporting symptoms of trimethylaminuria. For each we determined the sequence of the entire coding region, plus 1.3 kb of flanking intronic and 2.5 kb of the upstream region of the FMO3 gene. The affect of upstream variants on transcription was determined with a reporter gene assay. Results Seventy‐eight subjects were diagnosed as suffering from trimethylaminuria, based on urinary excretion of <90% of total TMA as TMA N ‐ oxide. Of these, 13 were classified as severe, 56 as moderate and nine as mild cases, excreting <43%, 48–70% and 73–83% of trimethylamine as trimethylamine N ‐oxide, respectively. Twenty‐seven mutations were identified in FMO3 , 15 in the coding region, of which eight abolish or severely impair FMO3 activity ( Pro70Leu , Cys197fsX , Thr201Lys , Arg205Cys , Met260Val , Trp388Ter , Gln470Ter and Arg500Ter ), and 12 in the upstream region. The mutations segregate into 19 haplotypes, including four different combinations of upstream mutations, each of which reduces transcriptional activity in comparison with the ancestral upstream sequence of FMO3 . Conclusions Comparisons of genotype and phenotype reveal that severe trimethylaminuria is caused by loss of function mutations in FMO3 . For moderate and mild cases the situation is more complex, with most resulting from factors other than FMO3 genotype. Our results have implications for the diagnosis and management of the disorder.