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The severity of footrot lesions induced by aprV2 ‐positive strains of Dichelobacter nodosus varies between strains
Author(s) -
Smith KJ,
Rosser MJ,
McPherson AS,
Whittington RJ,
Dhand NK,
Dhungyel OP
Publication year - 2021
Publication title -
australian veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.382
H-Index - 59
eISSN - 1751-0813
pISSN - 0005-0423
DOI - 10.1111/avj.13065
Subject(s) - virulence , biology , microbiology and biotechnology , foot rot , strain (injury) , gene , botany , anatomy , biochemistry
Objectives The primary objective of this study was to evaluate the clinical virulence of aprV2‐ positive lesser virulent field isolates of footrot bacteria Dichelobacter nodosus in comparison with an aprV2 ‐positive clinically virulent reference strain. Correlations between the clinical expression of the disease and the presence of aprV2 (detected using PCR tests) have been inconsistent. A second objective was to evaluate the elimination of D. nodosus following treatment of sheep as some strains of D. nodosus have been reported to be difficult to eliminate. Methods The virulence of three aprV2‐ positive field isolates of D. nodosus which had lesser virulent phenotypes, and an aprV2 ‐positive virulent reference strain was evaluated in a sheep trial using a pasture‐based experimental infection model. In the second phase of the study, treatments including footbathing and a long‐acting antibiotic were administered and their efficacy in elimination of these strains was evaluated. Results Severe underrun (score 4) lesions developed in sheep infected with the aprV2 ‐positive virulent reference strain but not in sheep infected with the field isolates; they had mild lesions (score 2 or 3). The three field isolates and the virulent reference strain of D. nodosus were eliminated by intensive foot bathing and antibiotic therapy in combination with housing the animals in dry conditions post‐treatment. Conclusion The results suggest that the presence of aprV2 gene in isolates of D. nodosus may not be a reliable indicator of virulence and that further investigation of the factors that determine clinical virulence is required. While the treatment regime was successful, based on a range of considerations, the use of such an intensive treatment involving antibiotics should be limited to small groups of high‐value animals, such as rams.