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Genome sequence of an Australian strain of canid alphaherpesvirus 1
Author(s) -
Sarker S,
Das S,
Helbig K,
Peters A,
Raidal SR
Publication year - 2017
Publication title -
australian veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.382
H-Index - 59
eISSN - 1751-0813
pISSN - 0005-0423
DOI - 10.1111/avj.12659
Subject(s) - genome , whole genome sequencing , biology , phylogenetic tree , genetics , strain (injury) , gene , dna sequencing , anatomy
Objective Characterisation of a complete genome sequence of an Australian strain of canid alphaherpesvirus 1 (CHV‐1) and its phylogenetic relationship with other varicellovirus species. Methods Standard pathology and PCR methods were used to initially detect herpesvirus in hepatic tissue from an infected 4‐week‐old Labrador Retriever puppy. The complete CHV‐1 genome was sequenced using next‐generation sequencing technology followed by de novo and reference assembly, and genome annotation. Results The CHV‐1 genome was 125 kbp in length and contained 74 predicted open reading frames encoding functional proteins, all of which have counterparts in other alphaherpesviruses. Phylogenetic analysis using the DNA polymerase gene revealed that the newly sequenced CHV‐1 clustered with canid alphaherpesvirus isolated from the UK and shared a 99% overall nucleotide sequence similarity. Conclusion This is the first complete genome of an Australian strain of CHV‐1, which will contribute to our understanding of the genetics and evolution of herpesvirus.