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Anti‐Müllerian hormone is expressed and secreted by bovine oviductal and endometrial epithelial cells
Author(s) -
Nasrin Ferdousy Raihana,
Kereilwe Onalenna,
Kadokawa Hiroya
Publication year - 2020
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/asj.13456
Subject(s) - oviduct , endometrium , paracrine signalling , uterine horns , ampulla , autocrine signalling , biology , endocrinology , medicine , estrous cycle , immunohistochemistry , andrology , hormone , uterus , receptor , anatomy
In this study, we investigated whether bovine oviducts and endometria produce anti‐Müllerian hormone (AMH) (for paracrine and autocrine signaling). Reverse transcription‐polymerase chain reaction and western blotting detected AMH expression in oviductal and endometrial specimens. Immunohistochemistry revealed robust AMH expression in the ampulla and isthmus epithelia, and the glandular and luminal endometrial epithelia (caruncular endometria). AMH mRNA (measured by real‐time PCR) and protein expression in these layers did not significantly differ among estrous phases in adult Japanese Black (JB) heifers ( p > .1). Furthermore, the expression in these layers also did not differ among Holstein cows (93.8 ± 5.8 months old), JB heifers (25.5 ± 0.4 months old), and JB cows (97.9 ± 7.9 months old). We also compared AMH concentrations in the oviduct and uterine horn fluids among the three groups (measured by immunoassays). Interestingly, the AMH concentration in the oviduct fluid, but not in the uterine horn fluid, of Holstein cows was lower than those in JB heifers and cows ( p < .05). Therefore, bovine oviducts and endometria express AMH and likely secrete it into the oviduct and uterine fluids.