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IL‐12p40 gene expression in lung and hilar lymph nodes of MPS‐resistant pigs
Author(s) -
Sakuma Akiko,
Sugawara Shizuka,
Hidaka Hikaru,
Nakajo Mitsuru,
Suda Yoshihito,
Shimazu Tomoyuki,
Rose Michael T.,
Urakawa Megumi,
Zhuang Tao,
Zhao Guoqi,
Watanabe Kouichi,
Nochi Tomonori,
Kitazawa Haruki,
Katoh Kazuo,
Suzuki Keiichi,
Aso Hisashi
Publication year - 2020
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/asj.13450
Subject(s) - lymph , lung , mycoplasma hyopneumoniae , biology , spleen , lesion , pathological , pneumonia , immunology , pathology , medicine , virology
Mycoplasma pneumonia of swine (MPS) is caused by Mycoplasma hyopneumoniae (M.hp) and is a common chronic respiratory disease of pigs. Recently, a genetically selected variant of the Landrace pig (Miyagino L2) has a lower incidence of pulmonary MPS lesions. We investigated the pathological and immunological characteristics of MPS resistance in these pigs ( n = 24) by comparing with the normal landrace pig (control: n = 24). The pathological MPS lung lesion score in MPS‐selected landrace pigs was significantly lower than in the control. The gene expression of interleukin (IL)‐12p40, which acts as a chemoattractant and a component of the bioactive cytokines IL‐12 and IL‐23, was significantly higher at the hilar lymph nodes, lung, and spleen in MPS‐selected landrace pigs than in control landrace pigs, and these were negatively correlated with the macroscopic MPS lung lesion score. In summary, we demonstrate that resistance against MPS in Miyagino L2 pigs is associated with IL‐12p40 up‐regulation, in comparison with normal landrace pigs without the MPS vaccine. In addition, a comparative study of macroscopic MPS lung lesions and IL‐12p40 gene expression in lung and hilar lymph nodes may lead to beneficial selection traits for the genetic selection for MPS resistance in pigs.