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Effects of early pregnancy on expression of interferon‐stimulated gene 15, STAT1, OAS1, MX1, and IP‐10 in ovine liver
Author(s) -
Yang Ling,
Li Ning,
Zhang Leying,
Bai Jiachen,
Zhao Zimo,
Wang Yujiao
Publication year - 2020
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/asj.13378
Subject(s) - isg15 , stat protein , stat1 , biology , interferon , interferon stimulated gene , western blot , pregnancy , downregulation and upregulation , andrology , stat5 , stat3 , gene expression , jak stat signaling pathway , messenger rna , medicine , gene , microbiology and biotechnology , immune system , immunology , signal transduction , innate immune system , genetics , ubiquitin , tyrosine kinase
Interferon‐tau (IFNT) regulates maternal recognition during early pregnancy in ruminants. The liver can serve as a hematopoietic organ, and it has immune functions. This study hypothesized whether mRNA and proteins of interferon‐stimulated genes (ISGs) induced by early pregnancy are upregulated in maternal liver. Therefore, we determined the expression of interferon‐stimulated gene 15‐kDa protein (ISG15), 2',5'‐oligoadenylate synthetase 1 (OAS1), myxovirus resistance protein 1 (MX1), interferon‐gamma‐inducible protein 10 (IP‐10), and signal transducer and activator of transcription 1 (STAT1) in maternal livers during early pregnancy in sheep. Ovine livers were sampled on day 16 of the estrous cycle, and days 13, 16, and 25 of pregnancy, and expression of ISGs was detected by quantitative real‐time PCR, Western blot, and immunohistochemistry analysis. Our results showed that there were increases in expression of the mRNA and proteins of ISG15, OAS1, IP‐10, STAT1, and MX1 during early pregnancy. STAT1 protein was limited to the hepatocytes, and endothelial cells of proper hepatic arteries and hepatic portal veins. In conclusion, the upregulation of ISG15, OAS1, IP‐10, STAT1, and MX1 proteins may be implicated in maternal hepatic immune adjustment and other functions during early pregnancy in sheep.