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Characteristic features of porcine endogenous retroviruses in Vietnamese native pigs
Author(s) -
Ishihara Shinya,
DangNguyen Thanh Q.,
Kikuchi Kazuhiro,
Arakawa Aisaku,
Mikawa Satoshi,
Osaki Makoto,
Otoi Takeshige,
Luu Quang Minh,
Nguyen Thanh Son,
Taniguchi Masaaki
Publication year - 2020
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/asj.13336
Subject(s) - endogenous retrovirus , biology , genetics , haplotype , gene , copy number variation , virology , genome , allele
We aimed to clarify the genomic characteristics of porcine endogenous retroviruses (PERVs) in Vietnamese native pig (VnP) breeds. First, we investigated genetic polymorphisms in β‐ and γ‐like PERVs, and we then measured the copy numbers of infectious γ‐like PERVs (PERV‐A, B, and C). We purified genomic DNA from 15 VnP breeds from 12 regions all over the country and three Western pig breeds as controls, and investigated genetic polymorphisms in all known PERVs, including the beta (β)1–4 and gamma (γ)1–5 groups. PERVs of β1, β2, β3, and γ4 were highly polymorphic with VnP‐specific haplotypes. We did not identify genetic polymorphisms in β4, γ1, or γ2 PERVs. We then applied a real‐time polymerase chain reaction–based method to estimate copy numbers of the gag , pol , and env genes of γ1 PERVs (defined as A, B, and C). VnP breeds showed significantly lower copy number of the PERV genes compared with the Western pig breeds (on average, 16.2 and 35.7 copies, respectively, p < .05). Two VnP breeds showed significantly higher copy number compared with the other VnPs ( p < .05). Our results elucidated that VnPs have specific haplotypes and a low copy number of PERV genes.