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Embryo collection from pigs post‐pseudopregnancy induced by estradiol dipropionate
Author(s) -
Hirayama Yuri,
Yoshioka Koji,
Noguchi Michiko,
Misumi Koji
Publication year - 2019
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/asj.13303
Subject(s) - estrous cycle , artificial insemination , equine chorionic gonadotropin , human chorionic gonadotropin , embryo , andrology , pregnancy , prostaglandin , intramuscular injection , medicine , embryo transfer , abortion , pregnancy rate , endocrinology , biology , ovulation , hormone , genetics , microbiology and biotechnology
We aimed to define whether embryo collection carried out after pseudopregnancy was of similar outcome and quality as after artificial abortion. To induce pseudopregnancy, 30 gilts or sows were given 20 mg intramuscular estradiol dipropionate (EDP) 10–11 days after the onset of estrus. Ten additional pigs were inseminated artificially at natural estrus as a control group. Prostaglandin F 2α (PGF 2α ) was administered twice with a 24 hr interval beginning 15, 20, or 25 days after EDP‐treatment ( n = 10 per group) or between 23 and 39 days after artificial insemination in control pigs. Following this, all pigs were given 1,000 IU equine chorionic gonadotropin and 500 IU human chorionic gonadotropin (hCG) and then inseminated. Embryos were recovered 6 or 7 days after hCG treatment and outcome was recorded. There was no significant difference in the number of normal embryos collected from the pigs with PGF 2α initiated at different time points or from the control group. Embryonic developmental stages 7 days after hCG treatment also did not differ among groups. These results indicate that the use of EDP to induce pseudopregnancy, followed by PGF 2α administration to synchronize estrus for subsequent embryo harvest, is a suitable alternative to the artificial abortion method.