Premium
Effect of melatonin on regulation of apoptosis and steroidogenesis in cultured buffalo granulosa cells
Author(s) -
Riaz Hasan,
Yousuf Muhammad Rizwan,
Liang Aixin,
Hua Guo Hua,
Yang Liguo
Publication year - 2019
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/asj.13152
Subject(s) - melatonin , melatonin receptor , medicine , endocrinology , receptor , apoptosis , downregulation and upregulation , messenger rna , hormone , biology , hormone receptor , chemistry , gene , cancer , biochemistry , breast cancer
This study was aimed to address melatonin receptor expression, mRNA level of hypothalamus and hypophysis hormone receptors (Gn RHR , FSHR , and LHR ), steroidogenesis, cell cycle, apoptosis, and their regulatory factors after addition of melatonin for 24 hr in cultured buffalo granulosa cells ( GC s). The results revealed that direct addition of different concentrations of melatonin (100 pM, 1 nM, and 100 nM) resulted in significant upregulation ( p < 0.05) of mRNA level of melatonin receptor 1a ( MT 1) without affecting melatonin receptor 1b ( MT 2). Melatonin treatment significantly downregulated ( p < 0.05) mRNA level of FSH and Gn RH receptors, whereas 100 nM dose of melatonin significantly increased mRNA level of LH receptor. Treatment with 100 nM of melatonin significantly decreased the basal progesterone production with significant decrease ( p < 0.05) in mRNA levels of St AR and p450ssc, and lower mRNA level of genes (Insig1, Lipe, and Scrab1) that affect cholesterol availability. Melatonin supplementation suppressed apoptosis (100 nM , p < 0.05) and enhanced G2/M phase (1 nM , 100 nM , p < 0.05) of cell cycle progression which was further corroborated by decrease in protein expression of caspase‐3, p21, and p27 and increase in bcl2. Our results demonstrate that melatonin regulates gonadotrophin receptors and ovarian steroidogenesis through MT 1. Furthermore, the notion of its incorporation in apoptosis and proliferation of buffalo GC s extends its role in buffalo ovaries.