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Immune synergistic oligodeoxynucleotide from Lactobacillus rhamnosus GG enhances the immune response upon co‐stimulation by bacterial and fungal cell wall components
Author(s) -
Nigar Shireen,
Yamamoto Yoshinari,
Okajima Takuma,
Sato Takashi,
Ogita Tasuku,
Shimosato Takeshi
Publication year - 2018
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/asj.13082
Subject(s) - immune system , lactobacillus rhamnosus , adjuvant , lipopolysaccharide , splenocyte , immunopotentiator , zymosan , microbiology and biotechnology , toll like receptor , biology , innate immune system , immunology , chemistry , bacteria , in vitro , lactobacillus , biochemistry , genetics
Bacterial genomic DNA has recently been shown to elicit a highly evolved immune defense. This response can be selectively triggered for a wide range of therapeutic applications, including use as a vaccine adjuvant to immunotherapies for allergy, cancer, and infectious diseases. Previously, we identified a low‐concentration immune synergistic oligodeoxynucleotide ( iSN ‐ ODN , named iSN 34) from Lactobacillus rhamnosus GG that has immunosynergistic activity upon costimulation of target cells with ligands of Toll‐like receptor 9 ( TLR 9). Here, we extend that observation by demonstrating the synergistic induction (in mouse splenocytes) of IL ‐6 by the combination of iSN 34 with cell wall components of bacteria and fungi. We observed that splenocytes pretreated with iSN 34 and then costimulated with agonists for TLR 1/2 (Pam 3 CSK 4 ), TLR 4 (lipopolysaccharide), or TLR 2/6 (Zymosan) exhibited enhanced accumulation of IL ‐6. These results suggested that the combination of iSN 34 with TLR 1/2, TLR 4, or TLR 2/6 agonists may permit the induction of a potent immune response.