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Changes of leukocyte counts and expression of pro‐ and anti‐inflammatory cytokines in peripheral leukocytes in periparturient dairy cows with retained fetal membranes
Author(s) -
Shimizu Takashi,
Morino Ikumi,
Kitaoka Ryuji,
Miyamoto Akio,
Kawashima Chiho,
Haneda Shingo,
Magata Fumie
Publication year - 2018
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/asj.13065
Subject(s) - peripheral blood mononuclear cell , andrology , inflammation , lymphocyte , medicine , tumor necrosis factor alpha , immunology , interleukin , fetus , postpartum period , endocrinology , cytokine , pregnancy , biology , in vitro , biochemistry , genetics
In dairy cows, retained fetal membranes (RFM) affect reproductive performance. The aim of this study was to examine the leukocyte counts and the gene expression of tumour necrosis factor α (TNFα), interleukin 1β (IL‐1β), IL‐8, and IL‐10 in polymorphonuclear leukocytes (PMNs) and peripheral blood mononuclear cells (PBMCs) in cows with ( n = 5) or without ( n = 5) RFM during the peripartum period. The lymphocyte counts in RFM cows were higher than those in control cows throughout the experiment ( p < .05). The expression of IL‐8 in PMNs of control cows was higher ( p < .05) compared with that of RFM cows postpartum. In cows with RFM, IL‐1β expression was higher ( p < .05) in PMNs at 6 weeks postpartum whereas the expression of IL‐1β was lower ( p < .05) in PBMCs at 4 weeks postpartum. The expression of IL‐10 in PBMCs of control cows was higher ( p < .05) than that of RFM cows at 2 weeks prepartum and 4 weeks postpartum. Taken together, our data indicate that changes of gene expression of pro‐ and anti‐inflammatory cytokines in RFM cows might be associated with the delayed placental separation and development of uterine inflammation in RFM cows.