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Effects of porcine brain hydrolysate on impairment of cognitive learning ability in amyloid β (1–40) ‐infused rats
Author(s) -
Liu YuTse,
Cheng FuYuan,
Takeda Shiro,
Lai KungMing,
Lin LiangChuan,
Sakata Ryoichi
Publication year - 2019
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/asj.13054
Subject(s) - lipid peroxidation , hydrolysate , acetylcholinesterase , chemistry , in vivo , malondialdehyde , aché , prolyl endopeptidase , pharmacology , neprilysin , biochemistry , endocrinology , medicine , oxidative stress , enzyme , biology , microbiology and biotechnology , hydrolysis
This study assessed whether administering porcine brain hydrolysate ( PBH ) ameliorates the impairment of spatial cognition learning ability in amyloid β (Aβ)‐infused rats. PBH was prepared using organic solvents (i.e., acetone and ethanol). Enzyme hydrolysates were derived from these PBH and the sequence of the Aβ peptide for infusion was selected. The results indicated the PBH , in particular EP (porcine brain extract with ethanol and protease N), demonstrated the potentials to reduce damage of neurodegenerative disorders in vitro and in vivo. The principal findings of this study indicate that PBH has prolyl endopeptidase inhibitory activity in vitro. Moreover, administering EP to Aβ(1–40)‐infused rats significantly improves their performance on reference, spatial performance, and working memory tests during water maze tasks; concurrent proportional decreases are also observed in malondialdehyde levels, acetylcholinesterase (AChE) activity, and Aβ accumulation levels in brain tissues. The PBH was suggested to ameliorate learning deficits associated with Alzheimer's disease by inhibition of lipid peroxidation in the brain of Aβ infused rat.